The following lab consists of a series of laboratory video clips and a laboratory report sheet that can be printed before you get started. When you have printed a copy of the lab sheet, view the test-tube contents video for test-tubes #1 to 7 and record the contents of each tube. Next you will view the test-tube liver reactions and record your observations. The Cool School Player can be used to compare reaction rates by using a frame by frame action. How many frames does it take for the oxygen bubbles to reach the top of the screen? Following your observations of each reaction, take a minute to develop an interpretation of why the reactants responded the way they did using the enzyme-substrate "lock and key" model of enzymatic action and your knowledge of the factors affecting enzyme activity. When you have completed the lab, view the explanations provided for each test-tube and include any additional information you might need in your lab notes. 

When you have completed this portion of the lab, you will be required to design an experiment that investigates the effect of pH, temperature, substrate concentration, or the addition of a competitive inhibitor on this enzyme catalyzed reaction.

The reaction taking place in the test tubes is the decomposition of 6% hydrogen peroxide into water and oxygen gas. Hydrogen peroxide is the substrate and water and oxygen gas are products. The reaction is catalyzed by the catalase enzyme found in liver.